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Subsequently, citrate is converted to isocitrate, which is then catalyzed by the rate-limiting enzyme isocitrate dehydrogenase to produce -ketoglutarate

doi: 10.1016/j.phrs.2022.106208 96

This study explains the preservation of sperm parameters in hypogonadal men on testosterone replacement therapy with concomitant low-dose human chorionic gonadotropin (hCG) Low-dose human chorionic gonadotropin maintains intratesticular testosterone in normal men with testosterone-induced gonadotropin suppression

Two tubes for each isolate were prepared and sub MIC of vitamin C was added to one of them

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Figure 1 Food-grade MSG manufactured by Ajinomoto was purchased commercially