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Bilirubin binding to MRGPRA1 was validated by competitive binding assays and bilirubin administration in Mrgpra1 and Blvra KO mice [34]

& Guillemin, R

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The protein complexes were then eluted with 100 L of elution buffer containing 50 mM BisTris, pH 6.8, 500 mM NaCl, and 500 mM imidazole and subjected to SDS-PAGE analysis

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