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The excision of the SAT1 flipper cassette was induced by inoculating 5 mL of YPD broth, containing 2% maltose (without nourseothricin), with the nourseothricin-resistant strains and incubating them in a rotary shaker at 30 C overnight

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121 Notably, patients with IDH mutant gliomas and concurrent TERTp mutations have poorer prognoses than those with IDH mutations alone, underscoring the complex molecular interactions governing glioma progression