In addition to bile acid metabolites, short-chain fatty acids (SCFAs), primarily composed of acetate (C2), propionate (C3), and butyrate (C4), are beneficial metabolites derived from the fermentation of dietary fiber and resistant starch by the gut microbiota
March 2, 2021
Speer, R
The membrane was blocked with 5% skim milk for 2 hours at room temperature and probed with the following antibodies: -Tubulin (1:500, ab6046, Abcam), SIRT5 (1:1000, ab259967, Abcam), SIRT3 (1:1000, ab246522, Abcam), SIRT4 (1:1000, #24662, Cell Signaling Technologies), SIRT1 (1:1000, ab189494, Abcam), COX IV (1:2000, ABL1060, Abbkine Scientific Co., Ltd., Wuhan, Hubei, China), Cleaved-caspase1 (1:1000, 89332, Cell Signaling Technologies), NLRP3 (1:1000, ab263899, Abcam), GSDMD-N (1:1000, 50928, Cell Signaling Technologies), Collagen (1:1000, ab255809, Abcam), Collagen (1:1000, PA5-34787, Thermo Fisher Scientific), GSTP1 (1:1000, 15902-1-AP, ProteinTech), SPI1 (1:1000, #2258, Cell Signaling Technologies), and Malonyl-Lysine [Mal-Lys] MultiMab Rabbit mAb mix (1:1000, #14942, Cell Signaling Technologies) overnight at 4C
Intracellular Mycobacterium avium intersect transferrin in the Rab11 + recycling endocytic pathway and avoid lipocalin 2 trafficking to the lysosomal pathway
ICP-MS CtAtm1 samples for which cluster was supplemented to purified protein as described above binding were purified using a 5 mL Hitrap desalting column (Cytiva) with buffer C (50 mM HEPES, pH = 8, 150 mM NaCl, 10% (v) glycerol, 5 mM BME, 2 mM MgCl 2 and 0.03% (w/v) DDM)