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Add cell lysis buffer (Catalogue No.:E050), collect the lysate solution (total protein concentration measured by BCA assay: 1.66 mg/ml with 20ug total protein loading) to detect.(no obvious band) Lane 2: Wild THP-1 cells were treated with 80nM TPA and cultured overnight, and then stimulated with 100ng/ml LPS for 6 hours, then cultured with 300ng/ml Brefeldin A (BFA) for 3 hours

Each TrxR1 and acTrxR1 enzyme variant (1 M) was incubated in phosphate buffer (100 mM potassium phosphate, 1 mM EDTA, pH 7.0) with increasing peroxide concentrations (0500 M H 2 O 2 (16,911-250ML-F, Sigma)) for 1 h in a total volume of 200 l at 37C

[46] Among these isolates, some marine yeasts originated from terrestrial habitats (grouped as facultative marine yeast), which were brought to and survived in marine environments

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doi: 10.1182/blood-2018-03-841932 33