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Loss of IL-1 K133 ubiquitylation does not impede IL-1 activation or release To explore if IL-1 K133 ubiquitylation and/or the K133:D129 electrostatic interaction also impacted Caspase-1-mediated cleavage and release of IL-1, we co-transfected 293T cells, which lack GSDMD expression 27 , with Caspase-1 and Il1b cDNAs

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Guo et al., 2016), the European Food Safety Authority estimated that the change of the urinary excretion rate of riboflavin corresponding to the point of tissue saturation could be achieved by a riboflavin intake of around 1.3mg/d (EFSA, 2017)