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The membranes were blocked with 5% BSA and incubated with primary antibodies at 4 C for 12 h, followed by secondary antibodies (HRP-conjugated Goat Anti-Mouse IgG, Proteintech, SA00001-1, 1:20000) for 1 h at room temperature

Do I have to reconstitute GHK-Cu myself

The peptide was shown to counteract the negative effects of methylprednisolone [9, 10]

By chelating iron, GHK-Cu provides an additional layer of cellular protection relevant to tissue health

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Due to their complex and stable structures, these dyes present a formidable challenge for degradation, posing harmful effects on humans, animals, and the environment with their inherent toxicity, mutagenicity, and carcinogenicity