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Its medicinal-chemistry program, described in detail by Kruse and colleagues, introduced several deliberate substitutions into the amylin backbone: residues borrowed from rat amylin (which does not readily form fibrils) to blunt the aggregation tendency, an engineered salt bridge to stabilize the central helix, and a C 20 fatty-diacid moiety attached through a linker to promote reversible binding to serum albumin

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Total RNA (2 g) was reverse transcribed into cDNA using the SuperScript III First-Strand Synthesis System (Invitrogen)

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As expected, the HbA1c level of OLETF rats was significantly higher than that of age-matched non-diabetic LETO rats (data not shown)