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The reads were processed and quality controlled using a malaria amplicon pipeline ( Markers with at least 10 coverage were considered to be successfully genotyped

Common mechanisms that play a role in developing GI disorders in patients with ASD include immune dysfunction, dietary metabolites, microbiota dysregulation, gut inflammation, dysautonomia and medication adverse reactions 12

Chromatographic separation was achieved using an Infinity Lab Poroshell 120 EC-C18 column (4.6 150 mm, 2.7 m particle size) and a binary gradient for the mobile phase (solvent A: water with 0.1% formic acid, and solvent B: acetonitrile with 0.1% formic acid)

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Gut 15 : 125129 Scheinberg DJ and Gitlin D (1952) Deficiency of ceruloplasmin in patients with hepatolenticular degeneration (Wilson's disease) Science 116 : 484485 Bull PC et al