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Based on our previous findings, we hypothesized that the metabolically active plasma exosomal CYP2E1 cargo participates in cellular pathophysiology and contributes to ALC- and APAP-induced toxicity in hepatic and non-hepatic cells

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(Kumar et al., 2016) created an injectable ultraviolet (UV) photocurable hydrogel containing poly-hydroxyethyl methacrylate (PHEMA), aminopropylmethacrylamide (APMA) and polyacrylic acid (PAA) to culture human BMSCs

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The cells were then washed using MACS buffer (0.5% BSA + 2mM EDTA in PBS) followed by staining with different clones of anti-CD38: LS198-4-2 (Beckman Coulter), JK36 (Beckman Coulter) and HIT2 (BD Biosciences) for 10 mins in the dark at room temperature