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Cells were harvested 72 h post-infection and stored at 80 C until processing

Abdallah CG, De Feyter HM, Averill LA, Jiang L, Averill CL, Chowdhury GMI, et al

It is synthesised in the body from the amino acids lysine and methionine, primarily in the liver and kidneys, and is also obtained through dietary sources such as red meat, fish, and dairy products

In a centrifuge tube layer 1 vol of blood over 1 vol of density barrier (see Figure 1 and Notes 2 and 3) and centrifuge at 350 g for 15 min at 20C in a swinging-bucket rotor and allow the rotor to decelerate without the brake

After 5 washes, 90-L of TMB substrate solution was added into each well, incubated for 20 min at 37 C

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