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Cells were lysed as previously described (Mukanganyama et al., Preparation of Alkylated GSTs Using iodo-APAP or IAM Reductive alkylation of human GSTA1, M1, M2, or P1 (1.83.6 nmol) was performed in 100 mM ammonium bicarbonate (ABC, pH 8.5) using DTT (250 nmol

El-Agnaf OMA, Salem SA, Paleologou KE, Cooper LJ, Fullwood NJ, Gibson MJ, et al

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Biomedicine & Pharmacotherapy , 58 , 168172

27 A small RCT found that adding gabapentin to physical therapy, bracing, and NSAIDs improved pain control in patients with lumbar spinal stenosis

However, the FDA also does not test supplements for safety or effectiveness