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Basic Calculation Framework To determine how much peptide is delivered per spray, use: Concentration (mcg/mL): Final solution strength after reconstitution (Convert if needed: mg/mL 1000 = mcg/mL) Spray Output (mL per actuation): Volume delivered per pump Formula mcg per spray = concentration spray volume Optional Unit-Based Conversion (U-100 Reference) If using insulin-style units as a volume reference: 1 mL = 100 units Spray volume (mL) = units 100 Updated Formula mcg per spray = concentration (units 100) Calculation Table (Template) Worked Examples (For Understanding Only) Example 1 (mL-based device): Concentration: 3333 mcg/mL (10 mg in 3 mL) Spray output: 0.10 mL mcg per spray = 3333 0.10 = 333.3 mcg Sprays per mL = 10 Total sprays (3 mL) = 30 sprays Example 2 (unit-based device): Concentration: 3333 mcg/mL Device output: 13 units Volume per spray = 13 100 = 0.13 mL mcg per spray = 3333 0.13 = 433.3 mcg Sprays per mL 7.7 Total sprays (3 mL) 23 sprays Final Note These examples are based on a 10 mg / 3 mL reconstitution for demonstration only

Having bacteriostatic water and acetic acid water available from a single UK source simplifies procurement for research laboratories handling a range of peptide types from water-soluble GLP-1 and amylin analogues that reconstitute directly in bacteriostatic water, to hydrophobic or basic peptides that require an acetic acid water first step before final dilution

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