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All animal experiments were approved by the Animal Ethics Committee of Qiqihar Medical University (Approval No.: 2022401)

Here's what to run, when to run it, and how to stack it based on four distinct goals

Wang X, Chen W, Li X, Zhou C, Deng C, Lv X, Fan Y, Men J, Liang C, Yu X

Then, cells were centrifuged and resuspended into 2 mL of prewarmed hypotonic solution (KCl 0.075 mmol L 1 ) and incubated in 14 mL of this solution for 15 min at 37 C