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Immunofluorescence staining of oocytes After a brief rinse with PBS/0.05% polyvinylpyrrolidone (to prevent adhesion), the oocytes were permeabilized with 0.5% Triton X-100/PHEM for 5 min, fixed in 3.7% PFA/PHEM (18.14 g PIPES, 6.5 g HEPES, 3.8 g EGTA, 0.99 g MgSO4, pH 7.0 w/KOH) for 20 min, and washed three times (10 min each) with PBS/polyvinylpyrrolidone

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Regulatory status: Neither peptide is FDA-approved, and both are banned for competitive athletes by WADA

About 85% of all CLN3 patients, including the patient participating in this study, are homozygous for the 1 kb deletion [40]

By contrast, the green fluorescence from AsGGT2 N100 -GFP was observed predominantly in the vacuole ( Figure 7B ), indicating that AsGGT2 has a signal sequence for targeting to the vacuole within its N-terminal 100 amino acid residues