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Hypermethylated CpG dinucleotides in TSGs are prone to spontaneous deamination of 5-methylcytosine (5mC) or to replication errors, resulting in C-to-T transitions that generate PTCs [6, 117]

Reversible aggregation of gold nanoparticles driven by inclusion complexation

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Since this SSE is associated with a loss of sensitivity and accuracy, the above-mentioned sample preparation methods describe compromises between the minimization of these matrix effects by removing matrix components during sample clean-up, and the ability to detect a broad spectrum of analytes with reasonable sample preparation efforts and adequate sensitivity

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