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Mitochondrial membrane potential was simultaneously probed using 1 M TMRM and Smart Fluo-Sensor Green, as described before [31, 39]

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For the non-interventional cohort, a group size of 20 subjects will detect a minimum difference in baseline GSH of at least 16% between young and elderly healthy subjects (0.05 alpha, 0.8 power) based on an inter-subject CV of 22%

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