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RNA-Seq Total RNA from three samples from each group (MOD, SEV and CTRL) at endpoints A and B (total 18 samples) was used to construct cDNA libraries with the TruSeq Stranded mRNA kit (Illumina, San Diego, CA) and the indexed fragments were sequenced on the NGS NextSeq 500 (Illumina) with the NextSeq 500/550 High Output 2x75 cycles kit (Illumina)

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If the preclinical data are promising, the drug sponsor can apply for an Investigational New Drug (IND) application to initiate clinical trials in humans

Better animal models are needed in order to recapitulate human disease, diverse antigenic history (mixed infection and vaccination) and immune phenomena such as original antigenic sin and imprinting