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In contrast, the processes of T cell activation, clonal expansion, differentiation into various effector T cell subsets, and subsequent effector function are characterized by increased energetic demands, resulting in the successive generation and replenishment of biosynthetic precursors that power rapid cell growth and proliferation (23, 28)

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After being blocked in the buffer (containing 0.5% Triton X-100, 5% donkey serum in PBS) for 1 hr at room temperature, they were incubated in primary antibodies, including anti-glutamate (1:500, rabbit, Sigma), anti-GABA (1:500, rabbit, Sigma), anti-ChAT (1:500, goat, Millipore),anti-mu opioid receptor (1:500, rabbit, Abcam), and anti-TUBB3 (1:500, rabbit, Abcam) at 4 C for 24 hr