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To confirm the MTT results, a replicate plate run in parallel was developed by adding 5 l of 2.5 M Calcein-AM (ThermoFisher Scientific) to selected wells and incubated 15 min at room temperature

The importance of nonaxonal pressure targets was accentuated in a landmark study by Morgans group, which demonstrated that pressure-induced dendritic retraction and RGC loss occur in the presence of intact axons (62)

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Examine the bag to ensure that the bag itself is intact and not leaking

However, tumor blood vessels are weak, leaky and fragile [9]

however, the sensitivity of this screening test is low, and the condition is often undiagnosed