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A 20 l aliquot of trypsinized cells was transferred to a microcentrifuge tube containing an equal amount of 0.4% trypan blue dye, mixed and loaded onto a hemocytometer

doi:10.1186/1471-2377-11-139 Tirouvanziam R, Obukhanych TV, Laval J, Aronov PA, Libove R, Banerjee AG, Parker KJ, O'Hara R, Herzenberg LA, Herzenberg LA, Hardan AY: Distinct plasma profile of polar neutral amino acids, leucine and glutamate in children with autism spectrum disorders

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To view a copy of this licence, visit About this article Cite this article Rus, C.P., de Vries, B.E.K., de Vries, I.E.J

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Studies have shown it modulates the expression of over 4,000 human genes shifting the expression profile toward a younger phenotype