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In these experiments, the thermal denaturation of the proteins is followed by monitoring the fluorescence enhancement of a probe (SYPRO Orange) that binds to protein hydrophobic patches upon denaturation process in the presence or absence of chemical compounds

doi: 10.1038/s41569-021-00541-4

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5) Gaps in ferroptosis and RCC metastasis: Ferroptosis is proposed to contribute to RCC metastasis (232), but the underlying mechanisms are largely unexplored

Using LCMS, the authors analyzed mixed-sex samples from ivermectin-resistant and susceptible strains and concluded that ivermectin resistance is closely associated with amino acid metabolism