CloneTracker™ 5-10 Single Barcode Cell Labeling Kit - 5-10 Unique Constructs + PCR Primers and Reagents CRISPR Cells BFP) as well as a

BFP) as well as a second construct with a non-target sgRNA and a second fluorescent marker (usually RFP or BFP)

sgRNA targeting the copGFP fluorescent protein in a standard (no Cas9) lentiviral vector

The DriverMap Mouse EXP Targeted Panel Kit includes all the enzymes

The CloneTracker XP barcode constructs are selectable by puromycin or hygromycin and a red fluorescent protein (RFP) marker

Gene List for Module 2: Cellecta Gene List 55K hGW shRNA Library M2

CloneTracker™ 5-10 Single Barcode Cell Labeling Kit - 5-10 Unique Constructs + PCR Primers and Reagents CRISPR Cells BFP) as well as aChoose 5 or 10 lentiviral constructs with barcodes flanked by common primer sequences to facilitate PCR amplifications. After transduction and antibiotic selection of cells, barcodes can be amplified and sequenced to estimate the number of cells present from a specifically labeled population. In this way, multiple cell populations can be labeled, mixed, growth together, and then the representative numbers of descendant cells from each population can