CRISPR sgRNA Vector with U6 Promoter and Cas9 (linearized, ready-for-cloning) Tetracycline-Inducible Systems and Vectors eLife 2016

eLife 2016

and Transduction

CMV_Cas9_Blast CRISPR pRSGCCBla-U6-sg-CMV-Cas9-2A-Blast SVCRU6CCN-L CMV_Cas9_Neo CRISPR pRSGCCN-U6-sg-CMV-Cas9-2A-Neo

000 constructs

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CRISPR sgRNA Vector with U6 Promoter and Cas9 (linearized, ready-for-cloning) Tetracycline-Inducible Systems and Vectors eLife 2016Vector that expresses the Cas9 protein and is designed for cloning and expression of sgRNA. Catalog # Description Vector SVCRU6CCP L CMV_Cas9_Puro CRISPR pRSGCCP U6 sg CMV Cas9 2A Puro SVCRU6CCH L CMV_Cas9_Hygro CRISPR pRSGCCH U6 sg CMV Cas9 2A Hygro SVCRU6CCB L CMV_Cas9_Blast CRISPR pRSGCCBla U6 sg CMV Cas9 2A Blast SVCRU6CCN L CMV_Cas9_Neo CRISPR pRSGCCN U6 sg CMV Cas9 2A Neo SVCRU6CCR L CMV_Cas9_RFP CRISPR pRSGCCR U6 sg CMV Cas9 2A RFP SVCRU6CCThy