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Bound FcRn was detected either by a horseradish peroxidase (HRP)-conjugated anti-GST antibody from goat (Rockland Immunochemicals, 200-301-200) (diluted 1:5000 in PBS/T/S) or streptavidin-AP (diluted 1:5000 in PBS/T/S) and visualized by addition of TMB substrate (CalBiochem) or p-nitrophenylphosphate (AP substrate) (Sigma-Aldrich) diluted to 10 g/mL in diethanolamine buffer

It only can be removed from the refrigerator at the injection time

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This may involve a detailed medical history review, a physical examination, and diagnostic tests like X-rays or Magnetic Resonance Imaging scans

Operators can set flow targets, monitor differential pressure, and initiate backwash sequences from a control room or mobile device