Cooling Coil stainless steel or plastic (Ultem

stainless steel or plastic (Ultem – Polyetherimide)

Simulating incubator conditions

Material: Acrylic

Hole size µm Percentage of open area 254 19 381 21 457 31 508 21

The Bio-Gel P-4 polyacrylamide beads are used at the time of Perifusion chamber setup

Cooling Coil stainless steel or plastic (UltemStainless steel cooling coils are used to lower the temperature of the digestive solution during the dilution phase. Cooling inactivates the collagenase to prevent healthy islets cells from being digested. This coil is used with a cold water bath or with the Ricordi Isolator Chiller. Individual coils are not sold sterile, but can be autoclaved prior to the first use. Biorep strongly suggests using a new coil for each isolation. Attempting to clean and