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Manual work is simple but can cause more errors and risk

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To test this, we first examined the effect of Cyss deprivation on the expression of exogenous full-length NRF2 (NRF2-FL) and NRF2-N89 and found that only the NRF2-N89 deletion mutant is insensitive to Cyss deprivation, confirming the involvement of KEAP1 in this regulation (Fig