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The determination of cell proliferation rate was based on the ratio of total and Ki67 + nuclei and the quantification of ICAM and VCAM expression per cell was calculated according to the formula CTCF (corrected total cell fluorescence) = integrated density (area of selected cell mean fluorescence of background reading), both using ImageJ software (imagej.nih.gov/ij/ )

These conversions result in the production of glucuronide and sulfate, curcumin compounds that render curcumin more bulky (which further limits curcumin uptake across the gut wall, meaning that a significant proportion of curcumin is excreted unabsorbed in the faeces) and water-soluble (allowing any absorbed curcumin to be easily excreted by the kidneys)

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