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When the cultures reached the mid-logarithmic phase (~0.60.8 OD), a 10-l aliquot was added to 2 ml of 10 mM sodium phosphate buffer (pH 7.4) containing 10% LB, 50 g ampicillin, and 10 g/ml hSP-A

These differences led to a significantly lower miscarriage rate and a notable 15-percentage-point increase in live birth rate compared to traditional smokers