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Fifty e-cigarette puffs was selected as an optimal controlled dose as it approximated the dose of nicotine delivered by the cigarette used in the study (~0.8 mg) based on machine yields of the e-cigarettes determined previously using a standardized Canadian Intense puffing protocol [20, 21]

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Repeated exposure to addictive substances can lead to changes in the brains structure and function

Author Freddy Sitas (Associate Professor, UNSW Sydney) emphasized that the evidence is already sufficiently consistent to support concern, even though definitive long-term epidemiological data will take decades to accumulate

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