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The research suggests they are putting themselves at heightened risk of unexplained weight loss, itchiness, increased thirst and overtiredness - all signs of type 2 diabetes.Sulakshan Neupane, lead author of the study, explained last month: "In an era when e-cigarettes are marketed as a 'safer' alternative to smoking, this suggests they may carry a hidden peril and may be quietly contributing to long-term health problems like prediabetes and diabetes

The supernatant was discarded and cell pellets were separately stained with the following staining solutions for different purposes as previously described [66,67,68]

Decreased daytime feelings of fatigue B

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