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Results Increased glucose uptake in cigarette smoking-associated EGFR TKI resistance in NSCLC To address the mechanism underlying cigarette smoke-triggered EGFR TKI resistance in EGFR WT NSCLC, H292 NSCLC cells, which express EGFR WT and are sensitive to EGFR TKI, were subjected to long-term treatments with CSE or benzo[]pyrene (B[]P), a human carcinogen found in tobacco smoke, for at least 3 months to mimic the behavior of chronic smokers with NSCLC [12, 17]

Also, studies have suggested that administration of nicotine in addition to reducing sperm number, motility, and survival can damage the cell membrane and sperm DNA and increase sperm production with abnormal morphology and finally induce apoptosis in testicular cells [39,40,41]

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