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But in real life, people might vape a liquid with nicotine one day and another without nicotine the next day

There are probably other preparations that the leaf goes through, but this one serves to define a significant difference

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Kar M, Emre IE, Muluk NB, Cingi C (2019) Effect of electronic cigarettes on the inner mucosa of the craniofacial region

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Additional analyses of data from both tasks that did not include the physiological noise modelling regressors produced a highly similar set of results (see Supplementary Figure 3), suggesting that physiological effects are not a significant confound in the data