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Membranes were treated with 5% BSA in PBS-T (PBS with 0.1% Tween 20) blocking buffer for at least 1 h at room temperature, then incubated with primary antibody diluted in blocking buffer overnight at 4C

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Une fois le matriel achet, un vapoteur confirm consommant 2 fioles de 10 ml et une rsistance par semaine dpensera donc aux alentours de 14 , alors qu'un fumeur confirm consommant 3 paquets par semaine dpensera, quant lui, aux alentours de 30

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