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Researchers theorize that when someone inhales nicotine through an e-cigarette or vape, they may consume a toxic level of nicotine, which may alter how the nicotine receptors respond

Nose-only exposures require some form of restraint, facilitating the study of the direct effects of inhalational exposure, but at the cost of significant stress on the animals (126a)

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Collectively, these findings demonstrate that the milieu in the lungs of cigarette smoke exposed mice promotes sustained chronic inflammation and hinders the accumulation of immune mediators and lymphocytes designed to combat bacterial infection