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HBEC were exposed to CS of different compositions and for different durations using an ISO3308 smoking regime and the impact of exposure was monitored in 2263 signaling pathways in the cell to generate a total effect score that reflects the quantitative degree of impact of external stimuli on the cells(Chen et al., 2021).Considering the fact that the concentration of smoke inhaled and the concentration of toxic substances in the blood cannot be controlled when people smoke, a set of instruments that can produce relatively stable and repeatable smoke concentrations is particularly important