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H1299 and GOT1/2 DKO H1299 cells expressing jAspSnFR3 and nuclear RFP were previously generated 22 , whereas wild-type (WT) 143B, GOT DKO 143B, and cells were engineered to express nuclear RFP (Cellomics, PLV-10205-50) and pLenti-jAspSnFR3 for this paper