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HTSeqFragments Per Kilobase of transcript per Million mapped reads (FPKM) gene expression matrices were log-transformed, and tumor-normal differential expression analyses were performed in R

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Making the expression privately is one thing, but I want to make very clear that Im not trying to take any space from anyone who has more on the line than me

The lysate was cleared by ultracentrifugation and batch-bound to HisPur Cobalt Resin (Thermo, 89964) equilibrated with binding buffer (20 mM Tris-HCl pH 7.2, 500 mM NaCl, 10 mM imidazole and 5% glycerol, pH adjusted to 8.0) for 30 min in the cold room with rocking

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