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10.1016/j.rmed.2015.06.009 [DOI] [PubMed] [Google Scholar] 79.Pandolfino JE, Kahrilas PJ

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This economic incentive to switch to less damaging products is important for people who already smoke, but also for people who are likely to take up nicotine use

The 60 L reaction mixture, containing 100 mM sodium phosphate buffer (pH 7.5), 1 mM EDTA, purified enzyme (150 g), and substrates (50 M10 mM), was initiated by enzyme addition and incubated at 37 C for 3 h