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The PCR reaction conditions included a pre-denaturation period of 3 min at 95 C, followed by denaturation for 15 s at 95 C, annealing for 15 s at 55 C, extension for 2 min at 72 C for 30 cycles, and finally an additional extension period for 5 min at 72 C

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One other aspect of the design deserves comment, which is the use of a purely baseline control condition in both tasks

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While itd be easy to assume as much from the massive AMG logos smeared across its flanks, this manned warhead of a motorboat is driven through the water by a pair of 9.0-liter Mercury Racing V8s that each generate 1,350 horsepower on 91-octane gasoline and 1,550 ponies on race fuel

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