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RNAScope FISH (tracheal digest cytospin labeling) Tracheal cells prepared for 10X scRNA-seq were distributed on slides via centrifuge at 55.3 g , fixed for 15 min in 3.2% PFA/1.5% sucrose at room temperature, rinsed with 1 PBS and stored at 4 C

Long-Term Effects of Inhalant Use Ulcers in the nose and mouth Frequent nosebleeds Runny nose Reduced appetite Weight loss Cognitive impairments Memory issues Lethargy Apathy Low mood Paranoia Blunted affect Heavy use comes with the risk of developing psychological conditions such as: Depression Anxiety Inhalant induced psychotic Neurocognitive disorders Inhalant intoxication delirium It can also lead to the following physical health problems: Asthma Damage to the lungs Sinusitis Bronchitis Tuberculosis Multi-organ damage Sudden death This kind of drug use can also have teratogenic effects, meaning it can cause changes to a foetus when used during pregnancy

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