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If you suspect your cat has ingested or inhaled weed, look for these signs: Vomiting Incoordination Dilated pupils Drooling Urinary incontinence Lethargy Increased vocalization Death is rare but not impossible

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S1: Dual visualisation of Nt TPC1A-GFP at the tonoplast and the plasma membrane, labelled by a brief pulse (~ 1 min) with 5 M of FM4-64 and quantification of cross-localisation of Nt TPC1A-GFP at the plasma membrane