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Western Blot Cells were lyzed with 0.3 ml of radioimmunoprecipitation assay buffer (50 mmol/l TrisHCl, 150 mmol/l NaCl, 1 mmol/l EDTA, 0.25% deoxycholate, 1 mmol/l NaF, 0.5 mmol/l Na 3 VO 4 , 1 mg/l aprotinin, 1 mg/l leupeptin, and 0.5 mmol/l phenylmethylsulfonyl fluoride)

You came back with a group of your friends, and we all stumbled out the backdoor and into the yard