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To further explore the unique immune cell changes across the different TLR KO mice in the context of NTHi-mediated inflammation, we studied the mRNA expression levels of different immune cell markers including total leukocytes ( CD45 ), T-cells ( CD3 ), neutrophils/granulocytes ( Ly6G ), cytotoxic T-cells ( CD8 ) and monocytes ( CD68 )

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