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Because e-cigarettes are perceived as less harmful and less dangerous than combustible cigarettes, another concern is that teens may be introduced to nicotine use via e-cigarettes, Barrington-Trimis said

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Cigarette smoke does not produce enough heat to trigger fire sprinklers

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Then, each slide was treated with 50 L of CMA3 staining solution and incubated in darkness for 30 min at room temperature (RT)