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The EDS Leiden Open Variation Database (LOVD) [27] contains approximately 200 and 60 unique COL5A1 and COL5A2 pathogenic variants, respectively

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On the day of capture, cells were quick thawed, washed with 1 PBS (no BSA) and counted before proceeding with WaferGen capture according to the manufacturers instructions with the following modifications: 5 or 10 10 4 cells were stained per sample, single-cell candidates were confirmed by manual visual triage