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Active ventilation (breathing) was generated by the ventilator, delivering breaths of a pressurized gas mixture to replicate the native lung environment (5% CO 2 , 21% O 2 , balance N 2 ) with independent controls for inspiratory time (set at 2 s), tidal volume (200 to 1200 mL) and respiratory rate (820 breaths per minute [BPM])

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