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Nicotine, particularly in those first few cigarettes, increases the activity of dopamine, a chemical in the brain that elicits pleasurable sensations

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Respiratory Issues: Combining tobacco with cannabis increases the risk of chronic obstructive pulmonary disease (COPD) and bronchitis

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The samples separated on the second principal component (8.1% of variance) of PCA plot, mainly due to lower relative abundances of Leptotrichia spp