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Lastly, to determine whether this impaired anti-bacterial defense was specific to menthol E-cig exposure, we measured ex vivo bacterial clearance by alveolar macrophages from mice that were exposed to traditional flavor (1.8% nicotine) E-cigs

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Next they connected these joints to a smoke cycle simulator machine that uniformly inhaled six times for three seconds each, then exhaled. Filters collected the aerosols at the machines 3-D-printed mouthpiece, and the researchers used analytical chemistry techniques to measure aerosol levels from puffs taken at the beginning, middle, and end of each joint

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