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Sample preparation for label-free quantitative MS Cells were lysed by addition of lysis buffer (8 M urea, 50 mM NaCl, 50 mM EPPS pH 8.5 and protease and Phosphatase inhibitors) and homogenization by bead beating (BioSpec) for three repeats of 30 s at 2,400 strokes per min

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[5] Angela McShane, Tobacco-Taking and Identity-Making in Early Modern Britain and North America, The Historical Journal 65, no

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A face-to-face interview conducted according to the DSM-IV interview schedule assessed the dependence on nicotine and the presence of a wide range of psychiatric disorders based on DSM-IV criteria

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